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rabbit anti p70 s6k  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit anti p70 s6k
    Rabbit Anti P70 S6k, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 4219 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+s6+kinase/p70+S6+Kinase+Antibody/pmc13016079-16-0-3
    Average 97 stars, based on 4219 article reviews
    rabbit anti p70 s6k - by Bioz Stars, 2026-10
    97/100 stars

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    Article Title: SPAG1 Inhibits Cell Proliferation and Tumor Growth of Lung Adenocarcinoma via the AKT/mTORC1 Signaling Axis
    Article Snippet: The primary antibodies used were as follows: rabbit anti-SPAG1 polyclonal antibody (cat. no. PA5-54879; dilution 1:800; Thermo Fisher Scienti c, Waltham, MA, USA); rabbit anti-AKT polyclonal antibody (cat. no. 9272; dilution 1:1,000; CST); rabbit anti-phosphorylated-AKT (cat. no. 4060; dilution 1:1,000; CST), rabbit anti-S6 kinase (cat. no. 2708; dilution 1:1,000; CST), mouse anti-phosphorylated-S6 kinase (cat. no. 9206; dilution 1:1,000; CST), rabbit anti-LC3B polyclonal antibody (cat. no. NB100‐2220; dilution 1:1,000; Novus Biologicals, LLC), rabbit anti-p62 polyclonal antibody (cat. no. 39749; dilution 1:1,000; CST) and mouse anti-β-actin monoclonal antibody (cat. no. TA811000; dilution 1:1,000; OriGene Technologies, Inc.).

    Article Title: Migfilin promotes autophagic flux through direct interaction with SNAP29 and Vamp8.
    Article Snippet: Autophagy plays a crucial role in cancer cell survival by facilitating the elimination of detrimental cellular components and the recycling of nutrients.. Understanding the molecular regulation of autophagy is critical for developing interventional approaches for cancer therapy.. In this study, we report that migfilin, a focal adhesion protein, plays a novel role in promoting autophagy by increasing autophagosome–lysosome fusion.

    Article Title: SPAG5 is associated with unfavorable prognosis in patients with lung adenocarcinoma and promotes proliferation, motility and autophagy in A549 cells
    Article Snippet: The primary antibodies used were as follows: Rabbit anti-SPAG5 polyclonal antibody [cat. no. 60940; 1:1,800; Cell Signaling Technology, Inc. (CST)], rabbit anti-caspase-3 polyclonal antibody (cat. no. 9662; 1:1,000; CST), rabbit anti-cleaved caspase-3 polyclonal antibody (cat. no. 9661; 1:500; CST), rabbit anti-poly(ADP-ribose) polymerase (PARP; cat. no. 9542; 1:1,000; CST), rabbit anti-S6 kinase (cat. no. 2708; 1:1,000; CST), mouse anti-phosphorylated-S6 kinase (cat. no. 9206; 1:1,000; CST), rabbit anti-light chain (LC) 3B polyclonal antibody (cat. no. NB100-2220; 1:1,000; Novus Biologicals, LLC), rabbit anti-p62 polyclonal antibody (cat. no. 39749; 1:1,000; CST) and mouse anti-β-actin monoclonal antibody (cat. no. TA811000; 1:1,000; OriGene Technologies, Inc.).

    Western Blot:

    Article Title: bFGF-mediated pluripotency maintenance in human induced pluripotent stem cells is associated with NRAS-MAPK signaling
    Article Snippet: Signals were visualized using ECL (enhanced chemiluminescence) reagent (GE Healthcare) and the Odyssey Fc Imaging System (LI-CORE Biosciences) respectively. .. The following antibodies were applied for immunoblotting: mouse anti-γ-tubulin (Sigma-Aldrich, T5326); mouse anti-OCT4 (Santa Cruz, sc-5279); rabbit anti-SOX2 (Invitrogen, PA1–16968); goat anti-NANOG (R&D systems, AF1997); mouse anti-SSEA4 (Millipore, MAB4304); mouse anti-α-SMA (DAKO, M0851); rabbit anti-GFAP (DAKO, Z0334); rabbit anti-MEK1/2 (#9126), rabbit anti-ERK1/2 (#9102), rabbit anti-RSK (#9355), rabbit anti-AKT (#9272), rabbit anti-p-MEK1/2 (S217/S221, #9154), rabbit anti-p-ERK1/2 (T202/T204, #9106), rabbit anti-p-p90RSK (T573, #9346), rabbit anti-p-AKT (S473, # 4060 and T308, #2965), rabbit anti-FOXO1 (#2880), rabbit anti-p-FOXO1 (S256, #9461), rabbit anti-S6 kinase (#2708), rabbit anti-p-p70 S6 kinase (T389, #9205), rabbit anti-p38 (#8690), rabbit anti-p-p38 (T180/Y182, #9211), rabbit anti-JNK (#9252), rabbit anti-p-JNK (T183/Y185, #9251), mouse anti-STAT3 (#9139S) and rabbit anti-p-STAT3 (Y705, #9145S) all from Cell Signaling. ..



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    | The MAS-transported NAD + /NADH regulates CAD phosphorylation activity and NDV replication through S6K. (A) Schematic diagram showing the potential involvement of mTOR-S6K in regulating CAD phosphorylation through MAS-mediated NAD + /NADH translocation. (B-D) A549 cells were either mock-infected or infected with NDV (MOI = 1) at 6, 12, 18 and 24 hpi. Protein levels of p-S6K <t>(T389),</t> S6K, p-S6 (S235/236), S6, p-CAD (S1859), CAD, NP and β-actin were analyzed by WB at 12 hpi (B). Grayscale quantification of p-S6K/S6K, p-S6/S6 and p-CAD/CAD ratios from WB data (C). Intracellular NAD + /NADH ratio were measured (D). (E-H) A549 cells were infected with NDV (MOI = 1), treated with AOA, supplemented with Pyr or Asp. Protein levels of p-S6K, S6K, p-S6, S6, p-CAD, CAD, NP, and β-actin were analyzed by WB at 12 hpi (E). Grayscale quantification of p-S6K/S6K, p-S6/S6 and p-CAD/CAD ratios from WB data (F). Intracellular NAD + /NADH ratio were measured (G). Extracellular virus yields were detected (H). (I-L) A549 cells were infected with NDV (MOI = 1), treated with AOA, supplemented with Pyr, and exposed to Torin 1. Protein levels of p-S6K, S6K, p-S6, S6, p-CAD, CAD, NP, and β-actin were analyzed by WB at 12 hpi (I). Grayscale quantification of p-S6K/S6K, p-S6/S6 and p-CAD/CAD ratios from WB data (J). Intracellular NAD + /NADH ratio were measured (K). Extracellular virus yields were detected (L). (M − P) A549 cells were infected with NDV (MOI = 1), treated with AOA, and supplemented with Duro. Protein levels of p-S6K, S6K, p-S6, S6, p-CAD, CAD, NP, and β-actin were analyzed by WB at 12 hpi (M). Grayscale quantification of p-S6K/S6K, p-S6/S6 and p-CAD/CAD ratios from WB data (N). Intracellular NAD + /NADH ratio were measured (O). Extracellular virus yields were detected (P). Data are presented as means from three independent experiments, ns, p > 0.05, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.
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    p70 s6 kinase  (Cell Signaling Technology Inc)
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    | The MAS-transported NAD + /NADH regulates CAD phosphorylation activity and NDV replication through S6K. (A) Schematic diagram showing the potential involvement of mTOR-S6K in regulating CAD phosphorylation through MAS-mediated NAD + /NADH translocation. (B-D) A549 cells were either mock-infected or infected with NDV (MOI = 1) at 6, 12, 18 and 24 hpi. Protein levels of p-S6K <t>(T389),</t> S6K, p-S6 (S235/236), S6, p-CAD (S1859), CAD, NP and β-actin were analyzed by WB at 12 hpi (B). Grayscale quantification of p-S6K/S6K, p-S6/S6 and p-CAD/CAD ratios from WB data (C). Intracellular NAD + /NADH ratio were measured (D). (E-H) A549 cells were infected with NDV (MOI = 1), treated with AOA, supplemented with Pyr or Asp. Protein levels of p-S6K, S6K, p-S6, S6, p-CAD, CAD, NP, and β-actin were analyzed by WB at 12 hpi (E). Grayscale quantification of p-S6K/S6K, p-S6/S6 and p-CAD/CAD ratios from WB data (F). Intracellular NAD + /NADH ratio were measured (G). Extracellular virus yields were detected (H). (I-L) A549 cells were infected with NDV (MOI = 1), treated with AOA, supplemented with Pyr, and exposed to Torin 1. Protein levels of p-S6K, S6K, p-S6, S6, p-CAD, CAD, NP, and β-actin were analyzed by WB at 12 hpi (I). Grayscale quantification of p-S6K/S6K, p-S6/S6 and p-CAD/CAD ratios from WB data (J). Intracellular NAD + /NADH ratio were measured (K). Extracellular virus yields were detected (L). (M − P) A549 cells were infected with NDV (MOI = 1), treated with AOA, and supplemented with Duro. Protein levels of p-S6K, S6K, p-S6, S6, p-CAD, CAD, NP, and β-actin were analyzed by WB at 12 hpi (M). Grayscale quantification of p-S6K/S6K, p-S6/S6 and p-CAD/CAD ratios from WB data (N). Intracellular NAD + /NADH ratio were measured (O). Extracellular virus yields were detected (P). Data are presented as means from three independent experiments, ns, p > 0.05, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.
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    | The MAS-transported NAD + /NADH regulates CAD phosphorylation activity and NDV replication through S6K. (A) Schematic diagram showing the potential involvement of mTOR-S6K in regulating CAD phosphorylation through MAS-mediated NAD + /NADH translocation. (B-D) A549 cells were either mock-infected or infected with NDV (MOI = 1) at 6, 12, 18 and 24 hpi. Protein levels of p-S6K (T389), S6K, p-S6 (S235/236), S6, p-CAD (S1859), CAD, NP and β-actin were analyzed by WB at 12 hpi (B). Grayscale quantification of p-S6K/S6K, p-S6/S6 and p-CAD/CAD ratios from WB data (C). Intracellular NAD + /NADH ratio were measured (D). (E-H) A549 cells were infected with NDV (MOI = 1), treated with AOA, supplemented with Pyr or Asp. Protein levels of p-S6K, S6K, p-S6, S6, p-CAD, CAD, NP, and β-actin were analyzed by WB at 12 hpi (E). Grayscale quantification of p-S6K/S6K, p-S6/S6 and p-CAD/CAD ratios from WB data (F). Intracellular NAD + /NADH ratio were measured (G). Extracellular virus yields were detected (H). (I-L) A549 cells were infected with NDV (MOI = 1), treated with AOA, supplemented with Pyr, and exposed to Torin 1. Protein levels of p-S6K, S6K, p-S6, S6, p-CAD, CAD, NP, and β-actin were analyzed by WB at 12 hpi (I). Grayscale quantification of p-S6K/S6K, p-S6/S6 and p-CAD/CAD ratios from WB data (J). Intracellular NAD + /NADH ratio were measured (K). Extracellular virus yields were detected (L). (M − P) A549 cells were infected with NDV (MOI = 1), treated with AOA, and supplemented with Duro. Protein levels of p-S6K, S6K, p-S6, S6, p-CAD, CAD, NP, and β-actin were analyzed by WB at 12 hpi (M). Grayscale quantification of p-S6K/S6K, p-S6/S6 and p-CAD/CAD ratios from WB data (N). Intracellular NAD + /NADH ratio were measured (O). Extracellular virus yields were detected (P). Data are presented as means from three independent experiments, ns, p > 0.05, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

    Journal: Tumour Virus Research

    Article Title: Oncolytic virus hijacks GOT1 and pyrimidinosomes to fuel pyrimidine synthesis for replication in tumor cells

    doi: 10.1016/j.tvr.2026.200342

    Figure Lengend Snippet: | The MAS-transported NAD + /NADH regulates CAD phosphorylation activity and NDV replication through S6K. (A) Schematic diagram showing the potential involvement of mTOR-S6K in regulating CAD phosphorylation through MAS-mediated NAD + /NADH translocation. (B-D) A549 cells were either mock-infected or infected with NDV (MOI = 1) at 6, 12, 18 and 24 hpi. Protein levels of p-S6K (T389), S6K, p-S6 (S235/236), S6, p-CAD (S1859), CAD, NP and β-actin were analyzed by WB at 12 hpi (B). Grayscale quantification of p-S6K/S6K, p-S6/S6 and p-CAD/CAD ratios from WB data (C). Intracellular NAD + /NADH ratio were measured (D). (E-H) A549 cells were infected with NDV (MOI = 1), treated with AOA, supplemented with Pyr or Asp. Protein levels of p-S6K, S6K, p-S6, S6, p-CAD, CAD, NP, and β-actin were analyzed by WB at 12 hpi (E). Grayscale quantification of p-S6K/S6K, p-S6/S6 and p-CAD/CAD ratios from WB data (F). Intracellular NAD + /NADH ratio were measured (G). Extracellular virus yields were detected (H). (I-L) A549 cells were infected with NDV (MOI = 1), treated with AOA, supplemented with Pyr, and exposed to Torin 1. Protein levels of p-S6K, S6K, p-S6, S6, p-CAD, CAD, NP, and β-actin were analyzed by WB at 12 hpi (I). Grayscale quantification of p-S6K/S6K, p-S6/S6 and p-CAD/CAD ratios from WB data (J). Intracellular NAD + /NADH ratio were measured (K). Extracellular virus yields were detected (L). (M − P) A549 cells were infected with NDV (MOI = 1), treated with AOA, and supplemented with Duro. Protein levels of p-S6K, S6K, p-S6, S6, p-CAD, CAD, NP, and β-actin were analyzed by WB at 12 hpi (M). Grayscale quantification of p-S6K/S6K, p-S6/S6 and p-CAD/CAD ratios from WB data (N). Intracellular NAD + /NADH ratio were measured (O). Extracellular virus yields were detected (P). Data are presented as means from three independent experiments, ns, p > 0.05, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

    Article Snippet: The antibodies used for immunoblotting in this study included monoclonal or polyclonal: anti-β-actin (Proteintech, Cat# 66009-1-Ig), anti-NP (Prepared in our laboratory), anti-S6 ribosomal protein (Cell Signaling Technology [CST], Cat# 2217), anti-phospho-S6 ribosomal protein (S235/236) (CST, Cat# 4858), anti-phospho-p70 S6 Kinase 1 (T389) (ABclonal, Cat# AP0564), anti-p70 S6 Kinase 1 (ABclonal, Cat# A2190), anti-CAD (CST, Cat# 11933), anti-phospho-CAD (S1859) (CST, Cat# 70307), anti-GOT1 (Proteintech, Cat# 14886-1-AP), anti-GOT2 (Proteintech, Cat# 14800-1-AP). anti-UMPS (Proteintech, Cat# 14830-1-AP), anti-DHODH (Proteintech, Cat# 14877-1-AP) and anti-Tom20 (Abcam, Cat# ab283317).

    Techniques: Phospho-proteomics, Activity Assay, Translocation Assay, Infection, Virus